@article{PAR00010519,
  title = {{P}artial disruption of translational and posttranslational machinery reshapes growth rates of {B}artonella birtlesii},
  author = {{R}olain, {J}. {M}. and {V}ayssier-{T}aussat, {M}. and {S}aisongkorh, {W}. and {M}erhej, {V}. and {G}imenez, {G}. and {R}obert, {C}. and {L}e {R}hun, {D}. and {D}ehio, {C}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{S}pecialization of bacteria in a new niche is associated with genome repertoire changes, and speciation in bacterial specialists is associated with genome reduction. {H}ere, we tested a signature-tagged mutant library of 3,456 {B}artonella birtlesii clones to detect mutants that could grow rapidly in vitro. {O}verall, we found 124 mutants that grew faster than the parental wild-type strain in vitro. {W}e sequenced the genomes of the four mutants with the most rapid growth (formed visible colonies in only 1 to 2 days compared with 5 days for the wild type) and compared them to the parental isolate genome. {W}e found that the number of disrupted genes associated with translation in the 124 rapid-growth clones was significantly higher than the number of genes involved in translation in the full genome ({P} < 10(-6)). {A}nalysis of transposon integration in the genome of the four most rapidly growing clones revealed that one clone lacked one of the two wild-type {RNA} ribosomal operons. {F}inally, one of the four clones did not induce bacteremia in our mouse model, whereas infection with the other three resulted in a significantly lower bacterial count in blood than that with the wild-type strain. {IMPORTANCE} {H}ere, we show that specialization in a specific niche could be caused by the disruption of critical genes. {M}ost of these genes were involved in translation, and we show that evolution of obligate parasitism bacteria was specifically associated with disruption of translation system-encoding genes.},
  keywords = {},
  booktitle = {},
  journal = {{M}bio},
  volume = {4},
  numero = {2},
  pages = {e00115--13},
  ISSN = {2150-7511},
  year = {2013},
  DOI = {10.1128/m{B}io.00115-13},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010519},
}