@article{PAR00010413,
  title = {{B}road spectrum of {M}imiviridae virophage allows its isolation using a {M}imivirus reporter},
  author = {{G}aia, {M}. and {P}agnier, {I}. and {C}ampocasso, {A}. and {F}ournous, {G}. and {R}aoult, {D}idier and {L}a {S}cola, {B}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he giant virus {M}imiviridae family includes 3 groups of viruses: group {A} (includes {A}canthamoeba polyphaga {M}imivirus), group {B} (includes {M}oumouvirus) and group {C} (includes {M}egavirus chilensis). {V}irophages have been isolated with both group {A} {M}imiviridae (the {M}amavirus strain) and the related {C}afeteria roenbergensis virus, and they have also been described by bioinformatic analysis of the {P}hycodnavirus. {H}ere, we found that the first two strains of virophages isolated with group {A} {M}imiviridae can multiply easily in groups {B} and {C} and play a role in gene transfer among these virus subgroups. {T}o isolate new virophages and their {M}imiviridae host in the environment, we used {PCR} to identify a sample with a virophage and a group {C} {M}imiviridae that failed to grow on amoeba. {M}oreover, we showed that virophages reduce the pathogenic effect of {M}imivirus (plaque formation), establishing its parasitic role on {M}imivirus. {W}e therefore developed a co-culture procedure using {A}canthamoeba polyphaga and {M}imivirus to recover the detected virophage and then sequenced the virophage's genome. {W}e present this technique as a novel approach to isolating virophages. {W}e demonstrated that the newly identified virophages replicate in the viral factories of all three groups of {M}imiviridae, suggesting that the spectrum of virophages is not limited to their initial host.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {4},
  pages = {e61912},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0061912},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010413},
}