@article{PAR00010412,
  title = {{P}lant and fungal diversity in gut microbiota as revealed by molecular and culture investigations},
  author = {{G}ouba, {N}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {F}ew studies describing eukaryotic communities in the human gut microbiota have been published. {T}he objective of this study was to investigate comprehensively the repertoire of plant and fungal species in the gut microbiota of an obese patient. {M}ethodology/{P}rincipal {F}indings: {A} stool specimen was collected from a 27-year-old {C}aucasian woman with a body mass index of 48.9 who was living in {M}arseille, {F}rance. {P}lant and fungal species were identified using a {PCR}-based method incorporating 25 primer pairs specific for each eukaryotic phylum and universal eukaryotic primers targeting 18{S} r{RNA}, internal transcribed spacer ({ITS}) and a chloroplast gene. {T}he {PCR} products amplified using these primers were cloned and sequenced. {T}hree different culture media were used to isolate fungi, and these cultured fungi were further identified by {ITS} sequencing. {A} total of 37 eukaryotic species were identified, including a {D}iatoms ({B}lastocystis sp.) species, 18 plant species from the {S}treptophyta phylum and 18 fungal species from the {A}scomycota, {B}asidiomycota and {C}hytridiocomycota phyla. {C}ultures yielded 16 fungal species, while {PCR}-sequencing identified 7 fungal species. {O}f these 7 species of fungi, 5 were also identified by culture. {T}wenty-one eukaryotic species were discovered for the first time in human gut microbiota, including 8 fungi ({A}spergillus flavipes, {B}eauveria bassiana, {I}saria farinosa, {P}enicillium brevicompactum, {P}enicillium dipodomyicola, {P}enicillium camemberti, {C}limacocystis sp. and {M}alassezia restricta). {M}any fungal species apparently originated from food, as did 11 plant species. {H}owever, four plant species ({A}tractylodes japonica, {F}ibraurea tinctoria, {A}ngelica anomala, {M}itella nuda) are used as medicinal plants. {C}onclusions/{S}ignificance: {I}nvestigating the eukaryotic components of gut microbiota may help us to understand their role in human health.},
  keywords = {{FRANCE}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {3},
  pages = {e59474},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0059474},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010412},
}