@article{PAR00010326,
  title = {{T}obacco {M}osaic {V}irus in the lungs of mice following intra-tracheal inoculation},
  author = {{B}alique, {F}. and {C}olson, {P}. and {B}arry, {A}. {O}. and {N}appez, {C}. and {F}erretti, {A}. and {A}l {M}oussawi, {K}. and {N}gounga, {T}. and {L}epidi, {H}. and {G}higo, {E}. and {M}ege, {J}. {L}. and {L}ecoq, {H}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lant viruses are generally considered incapable of infecting vertebrates. {A}ccordingly, they are not considered harmful for humans. {H}owever, a few studies questioned the certainty of this paradigm. {T}obacco mosaic virus ({TMV}) {RNA} has been detected in human samples and {TMV} {RNA} translation has been described in animal cells. {W}e sought to determine if {TMV} is detectable, persists, and remains viable in the lung tissues of mice following intratracheal inoculation, and we attempted to inoculate mouse macrophages with {TMV}. {I}n the animal model, mice were intratracheally inoculated with 10(11) viral particles and were sacrificed at different time points. {T}he virus was detected in the mouse lungs using immunohistochemistry, electron microscopy, real-time {RT}-{PCR} and sequencing, and its viability was studied with an infectivity assay on plants. {I}n the cellular model, the culture medium of murine bone marrow derived macrophages ({BMDM}) was inoculated with different concentrations of {TMV}, and the virus was detected with real-time {RT}-{PCR} and immunofluorescence. {I}n addition, anti-{TMV} antibodies were detected in mouse sera with {ELISA}. {W}e showed that infectious {TMV} could enter and persist in mouse lungs via the intratracheal route. {O}ver 14 days, the {TMV} {RNA} level decreased by 5 log(10) copies/ml in the mouse lungs and by 3.5 log(10) in macrophages recovered from bronchoalveolar lavage. {TMV} was localized to lung tissue, and its infectivity was observed on plants until 3 days after inoculation. {I}n addition, anti-{TMV} antibody seroconversions were observed in the sera from mice 7 days after inoculation. {I}n the cellular model, we observed that {TMV} persisted over 15 days after inoculation and it was visualized in the cytoplasm of the {BMDM}. {T}his work shows that a plant virus, {T}obacco mosaic virus, could persist and enter in cells in mammals, which raises questions about the potential interactions between {TMV} and human hosts.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {1},
  pages = {e54993},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0054993},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010326},
}