%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Drali, R.
%A Boutellis, A.
%A Raoult, Didier
%A Rolain, J. M.
%A Brouqui, P.
%T Distinguishing body lice from head lice by multiplex real-time PCR analysis of the Phum_PHUM540560 gene
%D 2013
%L PAR00010323
%G ENG
%J Plos One
%@ 1932-6203
%M ISI:000315524900236
%N 2
%P e58088
%R 10.1371/journal.pone.0058088
%U https://www.documentation.ird.fr/hor/PAR00010323
%V 8
%W Horizon (IRD)
%X Background: Body louse or head louse? Once removed from their environment, body and head lice are indistinguishable. Neither the morphological criteria used since the mid-18th century nor the various genetic studies conducted since the advent of molecular biology tools have allowed body lice and head lice to be differentiated. In this work, using a portion of the Phum_PHUM540560 gene from the body louse, we aimed to develop a multiplex real-time polymerase chain reaction (PCR) assay to differentiate between body and head lice in a single reaction. Materials and Methods: A total of 142 human lice were collected from mono-infested hosts from 13 countries on five continents. We first identified the louse clade using a cytochrome b (CYTB) PCR sequence alignment. We then aligned a fragment of the Phum_PHUM540560 gene amplified from head and body lice to design-specific TaqMan (R) FAM- and VIC-labeled probes. Results: All the analyzed lice were Clade A lice. A total of 22 polymorphisms between the body and head lice were characterized. The multiplex real-time PCR analysis enabled the body and head lice to be distinguished in two hours. This method is simple, with 100% specificity and sensitivity. Conclusions: We confirmed that the Phum_PHUM540560 gene is a useful genetic marker for the study of lice.
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