@article{PAR00010323,
  title = {{D}istinguishing body lice from head lice by multiplex real-time {PCR} analysis of the {P}hum_{PHUM}540560 gene},
  author = {{D}rali, {R}. and {B}outellis, {A}. and {R}aoult, {D}idier and {R}olain, {J}. {M}. and {B}rouqui, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {B}ody louse or head louse? {O}nce removed from their environment, body and head lice are indistinguishable. {N}either the morphological criteria used since the mid-18th century nor the various genetic studies conducted since the advent of molecular biology tools have allowed body lice and head lice to be differentiated. {I}n this work, using a portion of the {P}hum_{PHUM}540560 gene from the body louse, we aimed to develop a multiplex real-time polymerase chain reaction ({PCR}) assay to differentiate between body and head lice in a single reaction. {M}aterials and {M}ethods: {A} total of 142 human lice were collected from mono-infested hosts from 13 countries on five continents. {W}e first identified the louse clade using a cytochrome b ({CYTB}) {PCR} sequence alignment. {W}e then aligned a fragment of the {P}hum_{PHUM}540560 gene amplified from head and body lice to design-specific {T}aq{M}an ({R}) {FAM}- and {VIC}-labeled probes. {R}esults: {A}ll the analyzed lice were {C}lade {A} lice. {A} total of 22 polymorphisms between the body and head lice were characterized. {T}he multiplex real-time {PCR} analysis enabled the body and head lice to be distinguished in two hours. {T}his method is simple, with 100% specificity and sensitivity. {C}onclusions: {W}e confirmed that the {P}hum_{PHUM}540560 gene is a useful genetic marker for the study of lice.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {8},
  numero = {2},
  pages = {e58088},
  ISSN = {1932-6203},
  year = {2013},
  DOI = {10.1371/journal.pone.0058088},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010323},
}