@article{PAR00010196,
  title = {{A}xenic culture of fastidious and intracellular bacteria},
  author = {{S}ingh, {S}. and {E}ldin, {C}. and {K}owalczewska, {M}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he culture of microorganisms has been the basis of microbiology. {H}owever, revolutionary tools such as metagenomics have made it possible to describe uncultivated bacteria, but several breakthroughs have occurred in culture leading to a revival of these techniques. {I}n this review we focus on new applications that have successfully cultivated previously uncultivated bacteria. {W}e also review the axenic cultivation of intracellular bacteria such as {T}ropheryma whipplei and {C}oxiella burnetii. {T}hese successes provide new tools for the design of axenic media for intracellular bacteria, such as {R}ickettsiae and {C}hlamydiae, or historically uncultivable pathogens, such as {M}ycobacterium leprae and {T}reponema pallidum. {T}he future axenic culture of these microorganisms will facilitate antibiotic susceptibility testing and will provide insight into their microbial ecology and pathogenicity.},
  keywords = {axenic medium ; fastidious bacteria ; intracellular bacteria ; complex ; microbiota ; growth ; culture},
  booktitle = {},
  journal = {{T}rends in {M}icrobiology},
  volume = {21},
  numero = {2},
  pages = {92--99},
  ISSN = {0966-842{X}},
  year = {2013},
  DOI = {10.1016/j.tim.2012.10.007},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00010196},
}