@article{PAR00004371,
  title = {{M}ycobacterium marseillense sp. nov., {M}ycobacterium timonense sp. nov. and {M}ycobacterium bouchedurhonense sp. nov., members of the {M}ycobacterium avium complex},
  author = {{B}en {S}alah, {I}. and {C}ayrou, {C}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  abstract = {{A}n rpo{B} sequence-based evaluation of 100 {M}ycobacterium avium complex ({MAC}) clinical isolates led to the identification of five respiratory tract isolates that were potential representatives of three novel {MAC} species. {D}istinctive phenotypic features of isolates 62863 and 5356591({T}) included a pseudomycelium morphology and both esterase and acid phosphatase activities. {T}hese two isolates exhibited sequence similarities of 99.8 % for the 16{S} r{RNA} gene, 86.3 and 86.1 % for 16{S}-23{S} r{RNA} gene internal transcribed spacer ({ITS}-1) sequence, 96.7 and 97.8 % for rpo{B} and 97.6 and 97.4% for hsp65, respectively, with the type strain of {M}ycobacterium chimaera, the most closely related species. {I}solates 3256799 and 5351974({T}) lacked alpha-mannosidase and beta-glucosidase activities. {T}hey exhibited sequence similarities of 99.6 % for the 16{S} r{RNA} gene, 90.1 and 90.4 % for {ITS}-1, 97.8 % for rpo{B} and 98.0 and 98.1 % for hsp65, respectively, with the type strain of {M}. chimaera, the most closely related species. {I}solate 4355387({T}) lacked urease and alpha-glucosidase activities, but it exhibited valine arylamidase, cystine arylamidase and acid phosphatase activities. {I}t had sequence similarities of 99.3 % for the 16{S} r{RNA} gene, 51.8 % for {ITS}-1, 97.1 % for rpo{B} and 97.8 % for hsp65 with the type strain of {M}ycobacterium colombiense, the most closely related species. {A} phylogenetic tree based on concatenated 16{S} r{RNA} gene, {ITS}-1, rpo{B} and hsp65 sequences showed the uniqueness of these five isolates as representatives of three novel species, with bootstrap values >= 95 % in all nodes. {O}n the basis of these phenotypic and genetic characteristics, these five isolates are proposed as representatives of three novel {MAC} species: {M}ycobacterium marseillense sp. nov., with strain 5356591({T}) (={CCUG} 56325({T}) ={CIP} 109828({T}) ={CSUR} {P}30({T})) as the type strain; {M}ycobacterium timonense sp. nov., with strain 5351974({T}) (={CCUG} 56329({T}) ={CIP} 109830({T}) ={CSUR} {P}32({T})) as the type strain; and {M}ycobacterium bouchedurhonense sp. nov., with strain 4355387({T}) (={CCUG} 56331({T}) ={CIP} 109827({T}) ={CSUR} {P}34({T})) as the type strain.},
  keywords = {},
  journal = {{I}nternational {J}ournal of {S}ystematic and {E}volutionary {M}icrobiology},
  volume = {59},
  numero = {{P}art 11},
  pages = {2803--2808},
  ISSN = {1466-5026},
  year = {2009},
  DOI = {10.1099/ijs.0.010637-0},
  URL = {http://www.documentation.ird.fr/hor/{PAR}00004371},
}